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Difco luria bertani lb medium
Luria Bertani Lb Medium, supplied by Difco, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lb+medium/bertani+broth+lb+luria/pm42271439-61-0-6
Average 86 stars, based on 1 article reviews
luria bertani lb medium - by Bioz Stars, 2026-09
86/100 stars

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Article Title: Regioselective biosynthesis of oligoamides as precursors for sequence-controlled co-polyamides
Article Snippet: LB agar, LB medium and Terrific Broth were purchased from BD Difco.

Transformation Assay:

Article Title: Modified polypeptide having xylanase activity
Article Snippet: PCR was performed using Eppendorf Mastercycler Nexus GX2, and the reaction conditions are as follows: Initial Denaturation—94° C., 2 min Denaturation—94° C., 20 sec Annealing—50° C., 10 sec Extension—72° C., 10 min (From denaturation to extension 30 cycle) Final Extension—72° C., 5 min The resulting seven variants were ligated using an In-Fusion HD cloning kit (Takara, Cat. No. 639650), and then transformed into an E. coli Dh5α strain, followed by sequencing to confirm sequence mutation. .. The E. coli Dh5α strains transformed with the variants prepared in Example 1 and the Op Xyn gene were each inoculated into a sterilized LB medium (BD Difco), and cultured for 24 hours at 37° C. and 180 rpm, and then the cells were recovered by centrifugation. ..

Article Title: Modified polypeptide having xylanase activity
Article Snippet: PCR was performed using Eppendorf Mastercycler Nexus GX2, and the reaction conditions are as follows: Initial Denaturation—94° C., 2 min Denaturation—94C, 20 sec Annealing—50° C., 10 sec Extension—72° C., 10 min (From denaturation to extension 30 cycle) Final Extension—72C, 5 min The resulting variants were ligated using an In-Fusion HD cloning kit (Takara, Cat. No. 639650), and then transformed into an E. coli Dh5α strain, followed by sequencing to confirm sequence mutation. .. The E. coli Dh5α strains transformed with the SDM variants prepared in Example 1 and the Op Xyn gene were each inoculated into a sterilized LB medium (BD Difco), and cultured for 24 hours at 37° C. and 180 rpm, and then the cells were recovered by centrifugation. ..

Article Title: Modified polypeptide having xylanase activity
Article Snippet: PCR was performed using Eppendorf Mastercycler Nexus GX2, and the reaction conditions are as follows: Initial Denaturation—9400, 2 min Denaturation—94C, 20 sec Annealing—50°, 10 sec Extension—72° C., 10 min (From denaturation to extension 30 cycle) Final Extension—72C, 5 min The resulting seven variants were ligated using an In-Fusion HD cloning kit (Takara, Cat. No. 639650), and then transformed into an E. coli Dh5α strain, followed by sequencing to confirm sequence mutation. .. The E. coli Dh5α strains transformed with the variants prepared in Example 3-1 and the Op Xyn gene were each inoculated into a sterilized LB medium (BD Difco), and cultured for 24 hours at 37° C. and 180 rpm, and then the cells were recovered by centrifugation. ..

Cell Culture:

Article Title: Modified polypeptide having xylanase activity
Article Snippet: PCR was performed using Eppendorf Mastercycler Nexus GX2, and the reaction conditions are as follows: Initial Denaturation—94° C., 2 min Denaturation—94° C., 20 sec Annealing—50° C., 10 sec Extension—72° C., 10 min (From denaturation to extension 30 cycle) Final Extension—72° C., 5 min The resulting seven variants were ligated using an In-Fusion HD cloning kit (Takara, Cat. No. 639650), and then transformed into an E. coli Dh5α strain, followed by sequencing to confirm sequence mutation. .. The E. coli Dh5α strains transformed with the variants prepared in Example 1 and the Op Xyn gene were each inoculated into a sterilized LB medium (BD Difco), and cultured for 24 hours at 37° C. and 180 rpm, and then the cells were recovered by centrifugation. ..

Article Title: Modified polypeptide having xylanase activity
Article Snippet: PCR was performed using Eppendorf Mastercycler Nexus GX2, and the reaction conditions are as follows: Initial Denaturation—94° C., 2 min Denaturation—94C, 20 sec Annealing—50° C., 10 sec Extension—72° C., 10 min (From denaturation to extension 30 cycle) Final Extension—72C, 5 min The resulting variants were ligated using an In-Fusion HD cloning kit (Takara, Cat. No. 639650), and then transformed into an E. coli Dh5α strain, followed by sequencing to confirm sequence mutation. .. The E. coli Dh5α strains transformed with the SDM variants prepared in Example 1 and the Op Xyn gene were each inoculated into a sterilized LB medium (BD Difco), and cultured for 24 hours at 37° C. and 180 rpm, and then the cells were recovered by centrifugation. ..

Article Title: Modified polypeptide having xylanase activity
Article Snippet: PCR was performed using Eppendorf Mastercycler Nexus GX2, and the reaction conditions are as follows: Initial Denaturation—9400, 2 min Denaturation—94C, 20 sec Annealing—50°, 10 sec Extension—72° C., 10 min (From denaturation to extension 30 cycle) Final Extension—72C, 5 min The resulting seven variants were ligated using an In-Fusion HD cloning kit (Takara, Cat. No. 639650), and then transformed into an E. coli Dh5α strain, followed by sequencing to confirm sequence mutation. .. The E. coli Dh5α strains transformed with the variants prepared in Example 3-1 and the Op Xyn gene were each inoculated into a sterilized LB medium (BD Difco), and cultured for 24 hours at 37° C. and 180 rpm, and then the cells were recovered by centrifugation. ..

Article Title: Antimicrobial agents based on metal-ion zeolite materials: a multivariate approach to microbial growth inhibition
Article Snippet: .. Bacteria were cultured in LB medium (Difco) at 37 °C and 200 rpm until the optical density at 600 nm (OD 600 ) reached 0.4–0.6. ..

Centrifugation:

Article Title: Modified polypeptide having xylanase activity
Article Snippet: PCR was performed using Eppendorf Mastercycler Nexus GX2, and the reaction conditions are as follows: Initial Denaturation—94° C., 2 min Denaturation—94° C., 20 sec Annealing—50° C., 10 sec Extension—72° C., 10 min (From denaturation to extension 30 cycle) Final Extension—72° C., 5 min The resulting seven variants were ligated using an In-Fusion HD cloning kit (Takara, Cat. No. 639650), and then transformed into an E. coli Dh5α strain, followed by sequencing to confirm sequence mutation. .. The E. coli Dh5α strains transformed with the variants prepared in Example 1 and the Op Xyn gene were each inoculated into a sterilized LB medium (BD Difco), and cultured for 24 hours at 37° C. and 180 rpm, and then the cells were recovered by centrifugation. ..

Article Title: Modified polypeptide having xylanase activity
Article Snippet: PCR was performed using Eppendorf Mastercycler Nexus GX2, and the reaction conditions are as follows: Initial Denaturation—94° C., 2 min Denaturation—94C, 20 sec Annealing—50° C., 10 sec Extension—72° C., 10 min (From denaturation to extension 30 cycle) Final Extension—72C, 5 min The resulting variants were ligated using an In-Fusion HD cloning kit (Takara, Cat. No. 639650), and then transformed into an E. coli Dh5α strain, followed by sequencing to confirm sequence mutation. .. The E. coli Dh5α strains transformed with the SDM variants prepared in Example 1 and the Op Xyn gene were each inoculated into a sterilized LB medium (BD Difco), and cultured for 24 hours at 37° C. and 180 rpm, and then the cells were recovered by centrifugation. ..

Article Title: Modified polypeptide having xylanase activity
Article Snippet: PCR was performed using Eppendorf Mastercycler Nexus GX2, and the reaction conditions are as follows: Initial Denaturation—9400, 2 min Denaturation—94C, 20 sec Annealing—50°, 10 sec Extension—72° C., 10 min (From denaturation to extension 30 cycle) Final Extension—72C, 5 min The resulting seven variants were ligated using an In-Fusion HD cloning kit (Takara, Cat. No. 639650), and then transformed into an E. coli Dh5α strain, followed by sequencing to confirm sequence mutation. .. The E. coli Dh5α strains transformed with the variants prepared in Example 3-1 and the Op Xyn gene were each inoculated into a sterilized LB medium (BD Difco), and cultured for 24 hours at 37° C. and 180 rpm, and then the cells were recovered by centrifugation. ..

Bacteria:

Article Title: Antimicrobial agents based on metal-ion zeolite materials: a multivariate approach to microbial growth inhibition
Article Snippet: .. Bacteria were cultured in LB medium (Difco) at 37 °C and 200 rpm until the optical density at 600 nm (OD 600 ) reached 0.4–0.6. ..



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